James, 29, sustained a minor calf problem in last week's 1-1 Premier League draw at Burnley and has not trained this week.
Everton have lost their last two home games against Leeds United and Manchester United.
"We have James out – he had a little problem in the game against Burnley," Ancelotti said at his pre-match news conference.
"He was not able to train this week, I think he will start to train next week. For this game, we don't want to take a risk.
"He had a charge on his calf muscle after the game against Burnley. Nothing special, but he was not able to train.
"We have to look at him after the game to see if he will be able to train and play against Leicester [City, on Wednesday]."
Everton boss Ancelotti ruled out captain Seamus Coleman and midfielder Fabian Delph, who had both hoped to feature, while Lucas Digne and Jean-Philippe Gbamin are longer-term absentees.
"I'm not used to complaining about the players who are not available," added the Italian. "The players who are available trained well and are in good condition.
"We understand that there will be an important moment and test, not only on Saturday but in this period of December.
"So, I think we arrive at the game with confidence and motivation."
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